Automatic Continuous Monitoring of Enzymic Activity in Column Effluent.

Schwartz et al. (1) have described the application of the continuous flow system of analysis, developed by Skeggs (2), for the automatic assay of enzymic activities involving the nicotinamide adenine dinucleotide cofactors [NAD (H) and NADP(H) ] .2 This paper describes a further development of this application to the continuous monitoring of column effluents. Enzymes which have activities involving the reduction or oxidation of these dinucleotide cofactors, or which can be coupled to such enzymes, can be conveniently located in column ef?luents, with greater than 90% of the effluent available for further use. Thus the time required for manual enzyme assay of individual fractions is eliminated.